FLOW CYTOMETRY

The immunophenotyping laboratory is equipped with a suite of state of the art ten colour three laser flow cytometers and utilises a sophisticated automated lysing process allowing interrogation of forty markers of differentiation diagnosing malignancies with a quick turnaround time.

The Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) coordinates, manages and guides laboratory scientists and clinicians towards a fully comprehensive, streamlined and rapid diagnostic and monitoring service to the patient. Our repertoire includes HIV Immune (CD4:CD8) and CD19 monitoring.

The SIHMDS diagnostic algorithm, drafted and updated by haematology oncology consultants includes the disciplines of immunophenotyping, cytogenetics, histology and molecular testing, covering acute leukaemia (myeloid, T-cell, B-cell), chronic leukaemia (T-CLL, B-CLL, B-PLL) and lymphoma (MCL, FL, HCL and other) on bodily fluids.

A dedicated SIHMDS reception exists where staff carry out pre-analytical work such as data entry, processing slides, lymphocyte subsets and calibrating analysers.

Morphological screenings, where level of work up is discussed, are attended by scientists and medical staff, resulting in dispatch of samples to correct disciplines and inputting data onto an integrated reporting system.

Customised panels are used, pre-prepared by the manufacturer with full QC and titre information. Panel contents are selected by scientists and clinical consultants from WHO’s ‘Classification: Tumours of Haematopoietic and Lymphoid Tissues’. All laboratory staff process malignancy samples and fully trained BMSs interpret and report onto LIS; authorisation includes consultants and laboratory staff.  The process allows competitive turnaround times for routine work and malignancy work with undiagnosed malignancies reported within a few hours. An integrated report for all disciplines with a joint conclusion is made and reported by consultant staff.

Laboratory staff are involved in verification processes, preparation of documents and training of new staff. Communication with clinicians exist with all grades of staff making this a vibrant, challenging and exciting place to work where all levels of expertise, both professional and personal are utilised.

We hold the IBMS pre- and post- registration training approval for completion of the IBMS Registration Portfolio and Specialist Diplomas as well as being accredited under Clinical Pathology Accreditation whilst transitioning to ISO 15189 under the programme of UKAS assessment schedules.

HSL is a progressive partnership between The Doctors Laboratory, Royal Free London NHS Foundation Trust (the Royal Free London) and University College London Hospitals NHS Foundation Trust (UCLH)

Health Services Laboratories
Health Services Laboratories
Health Services Laboratories